Journal: Ophthalmology Science

Article Title: Cefiderocol Is an Effective Topical Monotherapy for Experimental Extensively Drug-Resistant Pseudomonas aeruginosa Keratitis

doi: 10.1016/j.xops.2023.100452

Figure Lengend Snippet: Susceptibility of Pseudomonas aeruginosa Strains Used in This Study

Article Snippet: To prepare cefiderocol, 1-g vials of the parenteral version of cefiderocol, FETROJA (Cefiderocol for Injection, Shionogi Inc) were used.

Techniques:

Journal: Ophthalmology Science

Article Title: Cefiderocol Is an Effective Topical Monotherapy for Experimental Extensively Drug-Resistant Pseudomonas aeruginosa Keratitis

doi: 10.1016/j.xops.2023.100452

Figure Lengend Snippet: Susceptibility of Pseudomonas aeruginosa Keratitis Isolates

Article Snippet: To prepare cefiderocol, 1-g vials of the parenteral version of cefiderocol, FETROJA (Cefiderocol for Injection, Shionogi Inc) were used.

Techniques:

Journal: Ophthalmology Science

Article Title: Cefiderocol Is an Effective Topical Monotherapy for Experimental Extensively Drug-Resistant Pseudomonas aeruginosa Keratitis

doi: 10.1016/j.xops.2023.100452

Figure Lengend Snippet: Susceptibility of Pseudomonas aeruginosa to selected antibiotics based on minimum inhibitory concentration values compared with Clinical and Laboratory Standards Institute break points. Asterisks indicated significant differences from cefiderocol group by chi-square with Yates correction. ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗∗ P < 0.0001.

Article Snippet: To prepare cefiderocol, 1-g vials of the parenteral version of cefiderocol, FETROJA (Cefiderocol for Injection, Shionogi Inc) were used.

Techniques: Concentration Assay

Journal: Ophthalmology Science

Article Title: Cefiderocol Is an Effective Topical Monotherapy for Experimental Extensively Drug-Resistant Pseudomonas aeruginosa Keratitis

doi: 10.1016/j.xops.2023.100452

Figure Lengend Snippet: Tolerability and behavioral impact of cefiderocol treatment. A, B , Toxicity scores (max 10 for conjunctival, 16 for corneal) of New Zealand White (NZW) rabbits exposed to topical drops of cefiderocol. Medians and interquartile ranges are shown, n = 6. No statistical differences were found between groups. C , Behavioral signs of NZW rabbits exposed to cefiderocol topical drops. Medians and interquartile ranges are shown, n = 39. Asterisks indicate significant differences from saline group by Kruskal–Wallis with Dunn’s post-test, P < 0.001.

Article Snippet: To prepare cefiderocol, 1-g vials of the parenteral version of cefiderocol, FETROJA (Cefiderocol for Injection, Shionogi Inc) were used.

Techniques: Saline

Journal: Ophthalmology Science

Article Title: Cefiderocol Is an Effective Topical Monotherapy for Experimental Extensively Drug-Resistant Pseudomonas aeruginosa Keratitis

doi: 10.1016/j.xops.2023.100452

Figure Lengend Snippet: Efficacy of antibacterial treatment. A, B , The colony-forming units (CFU) per cornea were determined by dilution plating. The onset group was evaluated at 16 hours postinfection, and before treatment. The other groups had 16 hours of untreated infection, followed by 8 hours of treatment as indicated. Asterisks indicated significant differences from the saline group by analysis of variance (ANOVA) with Tukey’s post-test. The pistachio lines represent a 99% and the red lines a 99.9% reduction in CFU compared with the onset. n = 6 except for onset group n ≥ 3. C, D , Images of eyes were taken before euthanasia from a fixed distance. The onset group was imaged at 16 hours postinfection, and before treatment. The other groups had 16 hours of untreated infection, followed by 8 hours of treatment as indicated. Relative infiltrate area was determined using ImageJ software. Mean and SD are shown and were compared by ANOVA and Tukey’s post-test. ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001; ∗∗∗∗ P < 0.0001. Panels A, C used strain K900, and panels B, D used strain CDC1270. CEF = cefiderocol; CIP = ciprofloxacin; TOB = tobramycin; XDR = extensively drug-resistant.

Article Snippet: To prepare cefiderocol, 1-g vials of the parenteral version of cefiderocol, FETROJA (Cefiderocol for Injection, Shionogi Inc) were used.

Techniques: Infection, Saline, Software

Journal: Ophthalmology Science

Article Title: Cefiderocol Is an Effective Topical Monotherapy for Experimental Extensively Drug-Resistant Pseudomonas aeruginosa Keratitis

doi: 10.1016/j.xops.2023.100452

Figure Lengend Snippet: The results of stability testing of refrigerated 50 mg/ml cefiderocol solutions. The means of 2 independent assays are shown.

Article Snippet: To prepare cefiderocol, 1-g vials of the parenteral version of cefiderocol, FETROJA (Cefiderocol for Injection, Shionogi Inc) were used.

Techniques: